From grape seeds, a
natural ally to improve
mood and stress
Grape (Vitis vinifera) belongs to family Vitaceae. Grapes itself is widely consumed all over the world. Major producers of grapes worldwide are USA, China, Italy and Europe.
Grapes are one of the most highly consumed fruits across the world. In ancient Europe the leaves and the sap of grape plants have been used in traditional treatment for ages. Besides being a wellspring for vitamins and fibers, the skin and seeds of grapes are highly rich in polyphenols specifically proanthocyanidins, which can be used as a functional ingredient to address various health issues by boosting the natural bio-processes of the body.
Proanthocyanidins are a large family of molecules existing in both monomeric and oligomeric structures up to more than 20 units. Many beneficial properties in cardiovascular, cancer, and neurological disorders as antioxidant, immunomodulatory, antidiabetic, cardio, and neuroprotective agents have long been described and recently reviewed.
Grape seeds are the part of the plant with the highest concentration of polyphenols, mainly oligomeric proanthocyanidins (OPCs), which have proven beneficial effects in cardiovascular health thanks to multiple mechanism of action.
ENOVITA® ORGANIC: the first Indena certified organic extract
Indena has 40 years experience in grape seed extract production: ENOVITA® ORGANIC is a 100% organic grape seed extract which comes from such a long and deep expertise and from an environmentally friendly approach to the production.
ENOVITA® ORGANIC is the first Indena organic extract, certified by Certipaq Bio sas in compliance with the European and USDA organic standards.
ENOVITA® is a proprietary proanthocyanidin (OPCs) rich extract, made exclusively with grape seeds from white wine production. Using only water as extraction solvent, ENOVITA® ORGANIC is a food grade grape seed extract. It has a double standardization: ≥95% of proanthocyanidins by spectrophotometry, ≥5% ≤15% of catechin and epicatechin HPLC.
As with all its botanical extracts, Indena manages the whole chain of ingredients’ life, from the raw material to the final production, to assure quality, safety and efficacy of the extract. To Indena, quality is indeed a comprehensive design approach to business as a whole, because today, one of the basic principles in quality management states quality cannot be merely tested in the final result, but should be built into products since the very beginning of manufacturing process.
Thanks to the collaboration with Alvinesa Natural Ingredients, Indena is able to offer a true sustainable product fully controlled along the value chain. The botanical raw material comes from farming fully compliant with the European and US organic regulations, from environmentally friendly process based on a full upcycling approach, that avoids the use of chemical fertilizers and pesticides and
up-cycles and transforms all vinification waste products. Moreover the extraction process of ENOVITA® ORGANIC is carried out using water and with no chemical solvents The grape seeds are sourced from vineyards in the Spanish South-Central region of Castilla-La Mancha, the largest organic vineyards in the world; then finally processed and released in Indena’s state-of-the art extraction plants, always in compliance with the EU and USDA organic standards.
ENOVITA® ORGANIC for quality of life: a surprising effect of Indena’s organic grape extract
A recent human study focused on the effects, both in vitro and in humans, of ENOVITA® on blood pressure revealed a surprising health counter-balance on perceived stress. It’s a randomized, double-blind, placebo-controlled study in healthy volunteers, which involved 80 healthy subjects, aged 40-70 years, 45 male and 35 female, with borderline and mild hypertension (125-140 mmHg systolic pressure measured for 7 days).
To check ENOVITA® effects on mood and stress parameters, the subjects received a Perceived Stress Questionnaire (PSQ) and Quality of life (SF-12) questionnaire.
The Perceived Stress Questionnaire (PSQ) is a validated tool to assess subjectively experienced stress independent of a specific or objective occasion. It consists of 4 domains (worries, tension, joy and demands) and has been filled by the involved subjects at the beginning of the study (baseline), after 8 weeks and after 16 weeks.
After 16 weeks with ENOVITA® supplementation, subjects experienced a significant reduction of worries: -17% vs baseline and – 26 % vs placebo, a reduction more pronounced in women than in men.
The Quality of Life Questionnaire Sf-12 – Mental Component Summary (MCS12) is a self-administered questionnaire containing 12 items and has been filled at baseline and after 16 weeks. ENOVITA® group reported a general increase of mental quality of life, more pronounced in women.
The statistically significant reduction of worries, as well as an improvement of the mental component score by trend, which could be noticed after ENOVITA® supplementation, indicates that a beneficial effect on stress and mood is associated with such a supplementation. An important result mainly in the current period, when resilience may help to face the stress due to the pandemic and its consequences. Future perspectives can be planned in order to explore in-depth the potential of ENOVITA® in the stress relaxing field, considering the positive unexpected results observed.
Blood pressure control in healthy people: ENOVITA® is the answer
ENOVITA® has already showed its effectiveness in modulating systolic and diastolic blood pressure in individuals prone to hypertension, thanks to a human study published in 2013.
The recent randomized, double-blind, placebo-controlled study in healthy volunteers showed that ENOVITA® modulated systolic blood pressure also in healthy population: the measured effect is in line with other RCT studies on grape seeds extracts, but it is now proven for the first time also in the challenging target of healthy subjects.
Such an evidence is significant considering the modern take on blood pressure, which is “the lower the better” because higher values are associated with higher cardiovascular risks. For this reason, recommended normal blood pressure values have progressively decreased over time and recent guidelines suggest to take care of blood pressure management in a much broader population, younger in age and with lower blood pressure values, which are traditionally more challenging to modulate.
ENOVITA® is the answer to the need for safe health food ingredients effective in supporting blood pressure control in healthy and borderline subjects.
ENOVITA® mechanism of action: the in-vitro study on endothelial dysfunction
The endothelium, a monolayer of endothelial cells, constitutes the inner cellular lining of the blood vessels (arteries, veins and capillaries) and the lymphatic system, being in direct contact with the blood/lymph and the circulating cells. Under homeostatic conditions, the endothelium maintains normal vascular tone and blood fluidity, by balancing the production of vasodilators (including nitric oxide NO) and vasoconstrictors.
Endothelial dysfunction is a broad alteration in endothelium structure and function and it is acknowledged as an early sign of cardiovascular disease.
The in-vitro study done in 20213 proved that ENOVITA® positively modulates endothelial function biomarkers (eNOS, endothelin-1 and sICAM). Those effects support the beneficial effects of ENOVITA® on endothelial healthy conditions.
2 Rauf, A.; Imran, M.; Abu-Izneid, T.; Haq, I.U.; Patel, S.; Pan, X.; Naz, S.; Silva, A.S.; Saeed, F.; Suleria, H.A.R. Proanthocyanidins: A comprehensive review. Biomed. Pharmacother. 2019, 116, 108999. https://www.sciencedirect.com/science/ article/pii/S0753332219305359?via%3Dihub
3 Christiane Schön et al., Grape Seed Extract Positively Modulates Blood Pressure and Perceived Stress: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Volunteers, Nutrients 2021, 13, 654. https://doi.org/10.3390/nu13020654 https://www.mdpi.com/journal/nutrients
4 Belcaro, Gianni, et al. “Grape seed procyanidins in pre-and mild hypertension: a registry study.” Evidence-Based Complementary and Alternative Medicine 2013 (2013)
5 Zhang, Haili, et al. “The impact of grape seed extract treatment on blood pressure changes: A meta-analysis of 16 randomized controlled trials.” Medicine 95.33 (2016).
6 Widlansky, Michael E., et al. “The clinical implications of endothelial dysfunction.” Journal of the American College of Cardiology 42.7 (2003): 1149-1160. – Bonetti, Piero O., Lilach O. Lerman, and Amir Lerman. “Endothelial dysfunction: a marker of atherosclerotic risk.” Arteriosclerosis, thrombosis, and vascular biology 23.2 (2003): 168-175.