Naticol® benefits in ageing

Anti-inflammatory activities of Naticol®

demonstrate benefits in ageing

Author: Dr. Christelle Bonnet

 

Ageing may be considered as the accumulation of diverse deleterious changes in cells and tissues (skin, joints…). One of the major changes that occur during ageing is the dysregulation of the immune response, leading to a chronic systemic inflammatory state. Among the dysregulated proinflammatory mediators, cytokines and chemokines are major culprits in the development of chronic inflammation and the immunosenescence process.

For instance, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and their receptors, are upregulated in aged tissues and cells (Bruunsgaard et al., 2003). Michaud et al. have shown that two to four-fold elevations in the circulating levels of inflammatory cytokines, such as TNF-α, IL-6, and IL-1β are typically observed in the elderly compared to the young.

Skin ageing answers to this chronic inflammatory state. Additionally, skin ageing, in particular, skin appearance being a primary indicator of age, with skin becoming unevenly coloured, lax, dry as it ages, a solution targeting skin appearance and specifically inflammation resolution seems relevant.

We believe collagen peptides can make an important difference in the way the skin looks and even in how well it ages. According to the literature, collagen peptides may slow down the collagen disorganization and destruction associated with dermal atrophy and skin aging. Scanning electron microscopic examination shows that the number and width of collagen fiber bundles decreases with age.

Naticol®, natural fish collagen peptides developed by Weishardt group is clinically substantiated to improve skin elasticity and reduce skin wrinkle appearance through an acceleration of collagen synthesis and inflammation resolution.

Within the joint, intra-articular administration of collagen in the knee in patients with osteoarthritis significantly decreases local inflammation (increase in T regs, IL-10 and decrease in IL-1b) and attenuates the symptoms inherent to the pathology (Furuzawa-Carballeda et al., 2012). Similarly, oral administration of collagen in mice with post-traumatic osteoarthritis inhibits synovial inflammation and induces cartilage regeneration (Dar et al., 2016). Naticol® has also clinically demonstrated its benefits in physical function to alleviate osteoarthritis symptoms. In metabolic disorders, it is suggested that the oral administration of collagen improves metabolism by directly targeting inflammatory processes (Astre et al., 2018).

Interestingly, it has also been shown that collagen hydrolysates inhibit zymosan- induced inflammation (Hartog et al., 2013).

In this article, we would like to point out how  Naticol® (specific fish collagen peptides) may exert its benefits in skin beauty  and articular comfort through an acceleration of collagen synthesis and anti-inflammatory activities.

In vitro benefits of Naticol® on matrix metalloprotease and collagen synthase expression

Human monocyte-derived macrophages (h-MDMs) and keratinocytes (HaCat) were co-cultivated in transwell culture system. The cells were stimulated with LPS (100ng/ml) to induce a
pro-inflammatory phenotype and then were traited by Naticol®.

The expression of Collagen Synthase 3 (enzyme involved in collagen production), of Metallopeptidase 13 (MMP13 involved in the breakdown of extracellular matrix and tissue remodeling), TGFb and IL-10 (anti-inflammatory and proresolutive cytokines) were evaluated by real-time qPCR.

Results showed a significant increase of the collagen synthesis through a decrease of the matrix metalloproteases and an increase of the collagen synthesis.

Naticol® and anti-inflammatory activities

Research has been conducted to determine the effects of  collagen peptides on inflammation. Indeed, it has been shown, in vivo, that MR (Mannose Receptor) specifically expressed by M2-like macrophages is responsible for the recognition and endocytosis of collagen across the fibronectin type II domain and recognizes both native collagen and hydrolyzed collagen peptides (Curino et al., 2005; Eichele et al.,2017; Engelholm et al., 2009;  Madsen et al., 2013; Malovic et al.,2007; Melander et al., 2015; Napper et al., 2006; Seikh et al. 2000). In our experiment, Naticol® was associated with lower levels of TNF-β and IL-1β pro-inflammatory cytokines. The attenuated inflammation by Naticol® treatment was also supported by induced TGFb and IL-10 anti-inflammatory cytokines secretion.

Anti-inflammatory properties of Naticol® have been demonstrated in pre-clinical studies. Consequently, Naticol® is the ideal ingredient delivering wellbeing and anti-ageing benefits.

Make your move in the anti-ageing category with Naticol®, specific fish collagen peptides from WEISHARDT !

 

References:

Astre G, Deleruyelle S, Dortignac A, Bonnet C, Valet P, Dray C. Diet-induced obesity and associated disorders are prevented by natural bioactive type 1 fish collagen peptides (Naticol®) treatment. J Physiol Biochem. nov 2018;74(4):647_54.

Bruunsgaard H, Ladelund S, Pedersen AN, Schroll M, Jørgensen T, Pedersen BK (2003). Predicting death from tumour necrosis factor-alpha and interleukin-6 in 80-year-old people. Clin Exp Immunol, 132: 24-31.

Curino AC, Engelholm LH, Yamada SS, Holmbeck K, Lund LR, Molinolo AA, et al. Intracellular collagen degradation mediated by uPARAP/Endo180 is a major pathway of extracellular matrix turnover during malignancy. J Cell Biol. 2005;169(6):977_85.

Dar Q-A, Maynard RD, Liu Z, Schott EM, Catheline S, Mooney RA, et al. Oral hydrolyzed type 1 collagen induces chondroregeneration and inhibits synovial inflammation in murine posttraumatic osteoarthritis. Osteoarthritis Cartilage. 2016;24:S532_3.

Eichele DD, Kharbanda KK. Dextran sodium sulfate colitis murine model: An indispensable tool for advancing our understanding of inflammatory bowel diseases pathogenesis. World J Gastroenterol. 7 sept 2017;23(33):6016_29.

Engelholm LH, Ingvarsen S, Jürgensen HJ, Hillig T, Madsen DH, Nielsen BS, et al. 2009;14:2103_14.

Furuzawa-Carballeda J, Lima G, Llorente L, Nuñez-Álvarez C, Ruiz-Ordaz BH, Echevarría-Zuno S, et al. Polymerized-type I collagen downregulates inflammation and improves clinical outcomes in patients with symptomatic knee osteoarthritis following arthroscopic lavage: a randomized, double-blind, and placebo-controlled clinical trial. ScientificWorldJournal. 2012;2012:342854.

Hartog A, Cozijnsen M, de Vrij G, Garssen J. Collagen hydrolysate inhibits zymosan-induced inflammation. Exp Biol Med Maywood NJ. 2013;238(7):798_802.

Madsen DH, Leonard D, Masedunskas A, Moyer A, Jürgensen HJ, Peters DE, et al. M2-like macrophages are responsible for collagen degradation through a mannose receptor-mediated pathway. J Cell Biol. 16 sept 2013;202(6):951_66.

Malovic I, Sørensen KK, Elvevold KH, Nedredal GI, Paulsen S, Erofeev AV, et al. The mannose receptor on murine liver sinusoidal endothelial cells is the main denatured collagen clearance receptor. Hepatol Baltim Md. juin 2007;45(6):1454_61.

Melander MC, Jürgensen HJ, Madsen DH, Engelholm LH, Behrendt N. The collagen receptor uPARAP/Endo180 in tissue degradation and cancer (Review). Int J Oncol. 2015;47(4):1177_88.

Michaud M, Balardy L, Moulis G, Gaudin C, Peyrot C, Vellas B, et al. (2013). Proinflammatory cytokines, aging, and age-related diseases. J Am Med Dir Assoc, 14: 877-882.

Napper CE, Drickamer K, Taylor ME. Collagen binding by the mannose receptor mediated through the fibronectin type II domain. Biochem J. 1 mai 2006;395(Pt 3):579_86.

Sheikh H, Yarwood H, Ashworth A, Isacke CM. Endo180, an endocytic recycling glycoprotein related to the macrophage mannose receptor is expressed on fibroblasts, endothelial cells and macrophages and functions as a lectin receptor. J Cell Sci. mars 2000;113 ( Pt 6):1021_32. 30

 

 

 

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